What is the intra-assay coefficient of variation

Determination of skatole and indole in back fat and muscle meat from pigs by high-performance liquid chromatography (HPLC) with fluorometric detection

Summary

This article describes a rapid and specific method for determination of 3-methylindole (skatole) and indole by high-performance liquid chromatography (HPLC) with fluorimetric detection in lean meat and backfat of pigs. The mean recovery of added skatole and indole was 104% and 106%, respectively, in backfat and in lean meat. The detection limit of the two compounds is approximately 0.005 μg / g. The mean coefficients of variation with this method are 5% for skatole and 10% for indole (intra-assay) and 6% for skatole and 11% for indole (inter-assay). The concentrations of skatole and indole were determined both in the backfat and the lean meat samples of boars used in examination. The results are specified comparatively.

Summary

A fast and specific high-performance liquid chromatography (HPLC) method with fluorometric detection is presented for the determination of 3-methylindole (skatole) and indole in meat and back fat from pigs. The mean recovery of added skatole and indole is 104% and 106%, respectively, in back fat and lean meat. The detection limit of the two substances is 0.005 μg / g. The mean coefficient of variation of the method was determined to be 5% for skatole and 10% for indole (intra-assay) and 6% or 11% (inter-assay). From the boar samples used for the investigation, the skatole and indole concentrations in both the fat and lean meat content were determined and compared.

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Author information

Affiliations

  1. Institute for Food Technology, Meat Technology Department (150), University of Hohenheim, Postfach 700562, W-7000, Stuttgart 70, Federal Republic of Germany

    Monika Gibis & Albert Fischer

  2. Institute for Animal Husbandry and Breeding (470), University of Hohenheim, Postfach 700562, W-7000, Stuttgart 70, Federal Republic of Germany

    Martin Dehnhard

Corresponding author

Correspondence to Albert Fischer.

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Gibis, M., Dehnhard, M. & Fischer, A. Determination of skatole and indole in back fat and muscle meat from pigs by high-performance liquid chromatography (HPLC) with fluorometric detection. Z Lebensm Unters Forch193, 220-223 (1991). https://doi.org/10.1007/BF01199969

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